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14.07.2023

Arg-His-Trp | A tripeptide and a beta-2 agonist

Researchers from India have discovered a tripeptide that may be of great interest to manufacturers of supplements for bodybuilders. According to in vitro and in silico studies, arginyl-histidyl-tryptophan is just as much of a full blown beta-2 agonist as clenbuterol.

Arg-His-Trp
In 2020, molecular scientist Hritik Chandore, affiliated with the Patil Biotechnology & Bioinformatics Institute in India, published a study in Protein and Peptide Letters in which he described a previously unknown effect of the tripeptide arginyl-histidyl-tryptophan [in short: Arg-His-Trp] [chemical structure below].

Chandore discovered the peptide in cells he cultured in vitro. The cells grew in fetal bovine serum. Fetal bovine serum is the blood of unborn calves, which is collected in slaughterhouses. Molecular scientists use it to grow cells, for which purpose fetal bovine serum is suitable as it contains many growth factors. Whether there are also minuscule bioactive proteins such as arginyl-histidyl-tryptophan in fetal bovine serum has never been investigated before.

The peptide came from the calf serum, Chandore discovered. It can apparently survive in the bloodstream.

Beta-2 adrenoceptor
Chandore also found that Arg-His-Trp interacted with the beta-2 adrenoceptor. That receptor is intended for adrenaline, and is used by substances such as clenbuterol and salmeterol. You can see below how, according to Chandore's calculations, Arg-His-Trp should interact with the beta-2 adrenoceptor.

The way in which Arg-His-Trp attaches to this receptor is quite robust. The biological effect of Arg-His-Trp should, again according to Chandore's calculations, be comparable to that of pharmacological beta-2 agonists.

Chandore suspects that this peptide can produce the same biological effects as pharmacological beta-2 agonists, but with fewer side effects.

Putting everything together
What do we have here? A substance consisting of three amino acids linked together. That shouldn't be exorbitantly expensive and companies should be able to market it without any problems.

Tripeptides are generally well absorbed. Because Arg-His-Trp probably also survives well in the bloodstream, the bioavailability should be perfectly fine. Toxic side effects such as liver and kidney damage are unlikely.

Beta-2 agonists help fight asthma and increase oxygen uptake. They also have a performance-enhancing effect and are suitable for improving body composition. They help to reduce fat mass while retaining muscle mass.

Argistropine - a trade name for Arg-His-Trp that we spontaneously come up with - could be a damn interesting sports supplement.

Source:
Protein Pept Lett. 2021;28(10):1191-1202.

More:
The clenbuterol effect of flax seed 01.01.2012
Phenol in cocoa has same effect as clenbuterol 13.12.2009

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